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世界*實驗材料供應商 Cell Technology正式授權上海起發為其中國代理， Cell Technology在一直是行業的*,一直為廣大科研客戶提供zui為的產品和服務,上海起發一直秉承為中國科研客戶帶來的產品，的服務，簽約 Cell Technology就是為了給廣大科研客戶帶來更加完善的產品和服務,您的滿意將是我們zui大的收獲
Cell Technology中國代理, Cell Technology上海代理， Cell Technology北京代理，Cell Technology廣東代理， Cell Technology江蘇代理Cell Technology湖北代理,Cell Technology天津,Cell Technology黑龍江代理,Cell Technology內蒙古代理,Cell Technology吉林代理,Cell Technology福建代理, Cell Technology江蘇代理, Cell Technology浙江代理, Cell Technology四川代理,
Cell Technology成立于1998年，致力于為研究院、生物技術公司及制藥企業的科研人員研發生產*的細胞功能研究用試劑與試劑盒。這些檢測試劑與試劑盒可以實現不破壞細胞形態和結構的情況下原位檢測細胞成分，研究細胞內發生的特定事件.Cell Technology發布了通過測定胞內活性caspase及線粒體膜電位研究細胞凋亡的一系列試劑。隨著公司研發團隊的持續努力，我們又推出了一系列高特異性的caspase檢測試劑，以及cathepsin檢測的試劑和ELISA試劑盒。目前Cell Technology提供的檢測試劑和ELISA試劑盒涵蓋Caspase、NOS、ROS、線粒體膜電位、信號通路和蛋白磷酸化等多個研究領域，產品倍各大高校、研究機構、生物技術企業和制藥企業采用。
Cell Technology主要產品簡介
1、Fluoro Cholesterol：高靈敏、一步檢測生物樣本中總膽固醇含量，可用于細胞代謝與藥物作用的研究。
2、Fluoro NADP：檢測組織或細胞提取液內NADP/NADPH含量，與NAD/NADH檢測無交叉，可用于細胞死亡、抗氧化/氧化應激、能量代謝及線粒體功能研究。
3、Fluoro Sarcosine：定量檢測細胞、血清及組織提取物中的肌氨酸含量。
4、Fluoro IndoBlu：定量檢測細胞中IndoBlu的含量，適用于細胞增殖研究。
5、Fluoro Lactate：定量檢測生物樣本中 L-lactate的含量。
6、Fluoro Phosphate：定量檢測生物樣本中的磷含量，靈敏度可達200nM。
Fluoro NADP
Fluorescent NADP/NADPH Detection Kit - Detection of NADP/NADPH in tissue extracts /cell samples
| Fluoro NADP™ NEW! Fluorescent NADP/NADPH Detection Kit - Detection of NADP/NADPH in tissue extracts /cell samples | |||||||||
| Key Benefits: | |||||||||
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| Introduction | |||||||||
Nicotinamide adenine dinucleotide phosphate (NADP+) is used in anabolic reactions, such as lipid and nucleic acid synthesis, which require NADPH as a reducing agent. NADPH is the reduced form of NADP+, and NADP+ is the oxidized form of NADPH. In cells, NADPH plays the role of a carrier of reducing power and is primarily involved in maintaining optimal redox metabolism. A simplified assay for the measurement of NAD and NADP is critical to understanding the roles of these pyridine nucleotides in normal and abnormal cells. | |||||||||
| Assay Principle | |||||||||
| The Fluoro NADP/NADPH detection kit utilizes a non-fluorescent detection reagent, which is oxidized in the presence NADPH to produce its fluorescent analog and NADP. NADP is further converted to NADPH via an enzyme-coupled reaction. The enzyme reaction specifically reacts with NADP/NADPH and not with NAD/NADH. Reaction: 1. NADPH + non-fluorescent detection reagent + electron coupler → fluorescent analog + NADP 2. NADP + enzyme coupled reaction → NADPH (then proceeds to reaction 1). Excitation: 530-570nm and Emission at 590-600nm | |||||||||
| References | |||||||||
| 1. Comprehensive Invited Review WEIHAI YING. NAD /NADH and NADP /NADPH in Cellular Functions and Cell Death: Regulation and Biological Consequences. ANTIOXIDANTS &REDOX SIGNALING Volume 10,Number 2,2008. 2. Bedard K and Krause KH. The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology.Physiol Rev 87:245 –313,2007. 3. Lowry, Oliver H., Passonneau, Janet V. and Rock, Martha K. The Stability of Pyridine Nucleotides. The Journal of Biological Chemistry, 236, #10, 1961. | |||||||||
| Kit contents (for 100 assays) | |||||||||
| 1. Enzyme Mix 170μL Part # 6022 .......................... 4-8C 2. NADP/NADPH Lysis Solution 12.5mL Part# 3050 ............................4-8C 3. NADP Extraction Buffer 22 mL Part# 3046 (Green label) ..........4-8C 4. NADPH Extraction Buffer 22 mL Part# 3047 (Magenta label)........4-8C 5. Reaction Buffer 11 mL. Part# 3049 ............................4-8C 6. Standard curve diluent 15 ml. Part # 3052............................4-8C 7. NADPH Detection reagent 110 μL Part # 4019...........................-20C 8. 3X Substrate Mix 6mL Part # 3048........................... -20C 9. NADPH Standard- 3 vials dried Part# 7014.............................-20C | |||||||||
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我們公司zui大優勢是強大的采購，
1：基本什么都能進口，血清，抗體，耗材，還有部分限制進口的，
2：貨品全，現經營過700多個品牌，基本所有生物試劑耗材都可以進口，特別是冷偏的產品那就更有優勢，
3：提供加急服務，一般1-2周到貨，超過時限加急費全免
4：價格公道，絕大部分價格有優勢，當然不能保證100%產品都是,因為意味著沒有服務.
5：良好的信譽，大部分客戶我們提供貨到付款服務，客戶包括清華，北大 交大 復旦，中山等100多所大學，ROCHE，阿斯利康，國藥 ，fisher等500多家公司
6：我們*代理的品牌有：Antibody Research Corporation，arcticzymes，Biorelevant，AmberGen, Inc. ，clemente-associates，clodronaiposomes，Columbia Biosciences，enzyme research，Gene Bridges GmbH，Genovis，AmberGen, Inc. Biotechnology GmbH，Haematologic Technologies HTI Haemtech，hookelabs，Immudex，Innovative Research of America，inspiralis ，List Biological Laboratories, Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-Freez Biologicals，pentapharm，progen，Protein Ark，QA-Bio,Inc，QA-Bio,IncQuickZyme Biosciences，Teknova，TriLink BioTechnologies, Inc.，Zyagen Laboratories 等
7：我們還是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批發，歡迎合作。
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